Equilibrium binding of alkyl isocyanides to human hemoglobin.

Equilibrium binding of alkyl isocyanides to human hemoglobin.

The reactions of human hemoglobin with a series of 13 alkyl isocyanides have been examined in equilibrium titration experiments at pH 7, 20 degrees C. The ligands include: methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, (+)- and (-)-sec-butyl, tert-butyl, n-pentyl, n-hexyl, cyclohexyl, and benzyl isocyanides. All of these compounds exhibit sigmoidal binding curves; however, the amount of...

The combining power of hemoglobin for alkyl isocyanides, and the nature of the heme-heme interactions in hemoglobin.

Technical Papers The Elucidation of Biocytin: Lemuel D. Wright et al. 635 The Function of the Cups of Polyporus conchifer: Harold J. Brodie ._ 636 Volatile Silica Affecting Plant Ash Analyses: Frank Liebenthal _ 636 Silicone Water-Repellents for General Use in the Laboratory: Paul T. Gilbert, Jr. _ 637 Multiplication of Rickettsia tsutsugamushi and Ornithosis Virus in Transplantable Mouse Tumor...

Nucleophilicity parameters for alkyl and aryl isocyanides.

Structural effects in alkyl nitrite oxidation of human hemoglobin.

Oxidations of hemoglobin in oxygen-saturated and deoxygenated media by 10 structurally variant alkyl nitrites have been examined in kinetic detail. Pronounced structural influences on rate constants, whose values span a range of 80 in oxidations of both oxy- and deoxyhemoglobin, have been observed. tert-Butyl nitrite provides the slowest oxidation rate that for deoxyhemoglobin terminates after ...

Manganese and Iron Binding to Human Transferrin

The characteristics of manganese and iron binding to human apotransferrin (apo-tf) have been investigated and compared in this study. Both metal ions were taken up by human apo-tf and formed complexes, with the maximum absorbances observed at 410 and 340 nm for manganese-transferrin (Mn-tf) and 465 nm for iron-transferrin (Fe-tf). Addition of manganese (1.5 µg/ml) to the reaction mixture contai...